ABSTRACT
Background: Autophagy, a cytosolic-structure degradation pathway, allows production of IL21 by CD4 T-cells and efficient cytolytic responses by CD8 T-cells. Autophagy is in part regulated by acyl-CoA-binding protein (ACBP) which has two functions. Intracellular ACBP favors autophagy, whereas secreted extracellular ACBP inhibits autophagy. Herein, we assessed whether autophagy and the ACBP pathway were associated with COVID-19 severity. Method(s): Through the BQC-19 Quebec biobank, somalogic proteomic analysis was performed on 5200 proteins in plasma samples collected between March 2020 and December 2021. Plasma from 903 patients (all data available) during the acute phase of COVID-19 were assessed. COVID-19 severity was stratified using WHO criteria. In vitro, ACBP intracellular levels, autophagy levels (LC3II) and IL21 production were assessed by flow in PBMCs after a 24h stimulation with IL6, phorbol myristate acetate (PMA)+ionomycin or lipopolysaccharide (LPS). Plasma levels of anti-SARS-CoV-2 (full spike protein or RBD) IgG were assessed by ELISA. Result(s): Median age of the cohort was 62 yo, 48% were female, 55% had comorbidities (see table). Increasing plasma levels of ACBP were found with severity (mild, moderate, severe and fatal groups having 5.3, 7.3, 9.5 and 10.6 RFU/50muL of plasma, respectively, p< 0.001 for all comparisons). Patients with comorbidities had higher plasma ACBP levels (7.4 vs 6.4 RFU/50muL, p< 0.001). Plasma ACBP levels were higher during the delta and omicron-variant periods (8.4 vs 6.8 RFU/50muL;p< 0.001). Plasma ACBP levels correlated with LC3II levels (r=0.51, P< 0.001) and IL6 (r=0.41, p< 0.001), but neither with markers IL1beta nor IL8. ACBP levels negatively correlated with IL21 levels (r=-0.27, p< 0.001), independently of age, sex, and severity. ACBP levels were not associated with levels of anti-SARS-CoV-2 IgG levels. In vitro, IL6 stimulation of healthy control PBMC induced extracellular ACBP release. Moreover, adding recombinant ACBP: 1) reduced autophagy in lymphocytes and monocytes upon polyclonal stimulation with PMA/ionomycin or LPS;2) reduced intracellular production of IL21 in T-cells after PMA/ ionomycin stimulation. Conclusion(s): Plasma ACBP levels were inversely linked with IL21 levels, suggesting that autophagy and IL21 allow control of SARS-CoV-2 infection, independently of the level of SARS-CoV-2 antibody secretion. ACBP is a targetable autophagy checkpoint and its extracellular inhibition may improve SARS-CoV-2 immune control. (Table Presented).
ABSTRACT
In 2020, the outbreak of COVID-19 pneumonia has had a great impact on China's economic and social life. The construction and transportation industries have been greatly impacted and suffered from its high mobility. This paper studies the big data of migration between Xi'an and Chengdu from January 1, 2020 to March 15, 2020 and divides the epidemic situation into four stages according to the introduction of the elastic coefficient according to the development of the epidemic situation. In each stage, the elastic coefficient of index change is introduced in combination with the decreasing impact of epidemic prevention and control measures on transportation. Finally, a modified moving average method is formed, which is compared with the ordinary moving average method. The results show that the modified moving average method combined with Hadoop big data platform can improve the accuracy and efficiency of the intercity transportation flow prediction under the epidemic situation. © 2021 CICTP 2021: Advanced Transportation, Enhanced Connection - Proceedings of the 21st COTA International Conference of Transportation Professionals. All rights reserved.
ABSTRACT
A large number of highly reactive disinfectants will be used in epidemic prevention of Corona Virus Disease-2019(COVID-19). In this paper, the application of highly reactive disinfectants was introduced, the occupational exposure hazards and mechanism of highly reactive disinfectants were analyzed, the preventive measures for occupational exposure hazards of highly active disinfectants and the use of specific neutralizing agents were proposed in order to prevent additional damage of staff health,equipment and environment caused by chlorine disinfectants, peracetic acid, glutaraldehyde and other highly reactive disinfectants,maintain the sustainability of disinfection operations,so as to play a positive role in epidemic prevention and control.